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Antimicrobial effect of synthesized tio2 nanoparticles against pathogenic microorganisms

Upasana Yadav, Manodeep Sen, Tanushri Chatterji and Qamar Rehman

Since many investigations into antimicrobial activity of titanium oxide have been published it still remains a particularly active research field. To our knowledge, the bactericidal properties of TiO2 synthesized by ball milling method have not been tested extensively. In this connection we synthesized TiO2 by ball milling method by using high-purity (99.9%) elemental powder of Ti of ~ 0.5 mm size. The powder was mechanically milled in a high-energy a ttritor ball mill (Szegvari Attritor) and examined its antibacterial properties. Antimicrobial action of as-prepared TiO2 was investigated using E. coli, P. aeruginosa and S. aureus. The bacterial growth was examined in the presence of TiO2, by the effect of UV light, and in the presence of both-TiO2 and UV light. The dynamics of antimicrobial action of the selected preparations was assessed by killing curves determination. The efficiency of antibacterial activity in standard experimental conditions as function of time was discussed. It can be concluded that the synthesized TiO2 (anatase) possesses strong antibacterial activity and could be used effectively for disinfection under UV illumination.

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